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The antibacterial activity of phillyrin on Escherichia coli ATCC8739 (E. coli ATCC8739),Bacillus subtilis BS08,Staphylococcus aureus ATCC6538 (S. aureus ATCC6538) and Salmonella ATCC14028 was determined by filter paper method. The...
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The antibacterial activity of phillyrin on Escherichia coli ATCC8739 (E. coli ATCC8739),Bacillus subtilis BS08,Staphylococcus aureus ATCC6538 (S. aureus ATCC6538) and Salmonella ATCC14028 was determined by filter paper method. The minimum inhibitory concentration (MIC) of the four tested bacteria was investigated by plate coating method to evaluate antibacterial ability. The antibacterial mechanism was further investigated by measuring growth curve,electric conductivity,nucleic acid content,Na+/K+-ATPase activity and polyacrylamide gel electrophoresis (SDS-PAGE). The results indicated that phillyrin had antibacterial effects and the MICs against Escherichia coli ATCC8739,Bacillus subtilis BS08,Staphylococcus aureus ATCC6538 and Salmonella ATCC14028 were 2.15,3.16,3.02 and 3.40 μg/mL respectively,and the antibacterial effect on Escherichia coli ATCC 8739 was more significant (p< 0.05) than that on other bacteria. Scanning electron microscopy (SEM) indicated that phillyrin destroyed the morphology of the cells and the cells ruptured. The leakage of intracellular substances led to an increase in nucleic acid content and an increase in electric conductivity in the bacterial suspension; SDS-PAGE analysis indicated that phillyrin could inhibit protein synthesis; in addition,phillyrin could reduce Na+/K+-ATPase activity. Therefore,phillyrin had obvious antibacterial ability and inhibited the expression of bacterial proteins by destroying the cell membrane structure,resulting in the death of the cells.
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Antibacterial nanocrystals have attracted great interests in recent years. In fact, with the emergence and increase of microbial organisms resistant to multiple antibiotics, many researchers have tried to develop new antibiotics. ...
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Antibacterial nanocrystals have attracted great interests in recent years. In fact, with the emergence and increase of microbial organisms resistant to multiple antibiotics, many researchers have tried to develop new antibiotics. The aim of this research is to compare antibacterial activity of mono-metallic with composite nanocrystals, againstEscherichia coli,?Salmonella galinarium, Staphylococcus aureus,?Pesudomonas aueroginosa,?and?Bacillus subtilis,?with and without?sonication, for the first time. Mono-metallic with composite nanocrystals, are synthesized via wet method and ensure with oxalate decomposition in high temperature (500°C). FT-IR, XRD, SEM were used for determination of spectroscopic, structural and morphology of samples, respectively. Also the nanoparticls were digested and analyzed by ICP-AES for determining the presence of residual chemical element in the nanoparticles, after sonication. Bacterial sensitivity to nanocrystals, with and without?sonication, were commonly tested using disc diffusion test and agar dilution test, also with determination of minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). The particles size was less of 100 nm, approximately. This study shows that synthesis of mono-metallic and composite nanocrystals, with oxalate decomposition method is simple and so useful. Although, we confirmed that utilized of the ultrasonic vibration were futile, entirely. Also, the Ag/ZnO nanocrystals have great antimicrobial agent against all of the strains and?just?combination of zinc oxide and silver?nanocrystals, give increase their bactericidal effect.
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A series of N-cyclohexylidene-N-phenylanines 3 are prepared by the condensation of cyclohexanone 1 with aryl amine 2, subsequent treatment of 3 with thiomalic acid give the corresponding 2-(3-oxo-4-aryl-1-thia-4-azaspiro[4.5]dec-2...
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A series of N-cyclohexylidene-N-phenylanines 3 are prepared by the condensation of cyclohexanone 1 with aryl amine 2, subsequent treatment of 3 with thiomalic acid give the corresponding 2-(3-oxo-4-aryl-1-thia-4-azaspiro[4.5]dec-2-yl)acetic acid 4, which on reaction with o-phenylenediamine give 2-(1H-benzo[d]imidazol-2-ylmethyl)-4-aryl-1-thia-4-azaspiro[4.5]decan-3-one 5. Characterization of all the compounds has been done by IR, H-1 NMR, MS and elemental analyses. The antibacterial, antifungal and nematicidal activities of the compounds have also been evaluated.
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A series of benzimidazole derivatives were synthesized for this investigation. NMR and IR spectrum analysis as well as carbon, hydrogen, and nitrogen studies were used to characterize these novel synthesized derivatives. Antibacte...
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A series of benzimidazole derivatives were synthesized for this investigation. NMR and IR spectrum analysis as well as carbon, hydrogen, and nitrogen studies were used to characterize these novel synthesized derivatives. Antibacterial and antifungal tests were conducted on all of the newly synthesized compounds. Ciprofloxacin and fluconazole were utilized as reference medications in antibacterial and antifungal research, respectively. The microbiological assay revealed that the compounds show promising antibacterial and antifungal activity.
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Among biological applications, plant-mediated Pd NPs for multi-drug resistance (MDR) developed in pathogenic bacteria were synthesized with the help of biomass of lemon peel, a biological material, with a non-toxic, environmentall...
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Among biological applications, plant-mediated Pd NPs for multi-drug resistance (MDR) developed in pathogenic bacteria were synthesized with the help of biomass of lemon peel, a biological material, with a non-toxic, environmentally friendly, human-nature green synthesis method. Characterization of synthesized Pd NPs was carried out by UV-Vis spectrometry, Transmissive Electron Microscopy (TEM), X-ray diffraction (XRD), and
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A bioactive compound with antibacterial activity was isolated and purified from the extract of leaves of sunflower, Helianthus annuus. The bioactive compound was characterized using H-1 and C-13 NMR. The compound induced auxin, gi...
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A bioactive compound with antibacterial activity was isolated and purified from the extract of leaves of sunflower, Helianthus annuus. The bioactive compound was characterized using H-1 and C-13 NMR. The compound induced auxin, gibberellins and cytokinin in Oryza sativa and Phaseolus mungo. It also showed activity against Xanthomonas oryzae pv. oryzae.
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Nicotine, isolated from leaves of?Nicotiana tabacum?was complexed with zinc and studied for their antibacterial activities against ten different strains of Gram positive and Gram negative bacteria. Results showed that zinc (II) co...
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Nicotine, isolated from leaves of?Nicotiana tabacum?was complexed with zinc and studied for their antibacterial activities against ten different strains of Gram positive and Gram negative bacteria. Results showed that zinc (II) complex of nicotine is more active against different types of bacterial strains as compared to zinc metal salt used for complexation and nicotine alone.?Key?words:?Antibacterial activities,?Nicotiana tabacum, nicotine, zinc (II) complex.
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Background: Methicillin resistant Staphylococcus aureus (MRSA) usually invalidate powerful antibiotics in the clinic. Pleuromutilin derivatives have been reported to possess antibacterial activity against MRSA.
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Madura has been known as an Indonesian tribe that usually used many recipes of traditional medicine in their daily life. One of the medicinal herbs to increase female fertility is the herbal "Subur Kandungan", which consists of ga...
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Madura has been known as an Indonesian tribe that usually used many recipes of traditional medicine in their daily life. One of the medicinal herbs to increase female fertility is the herbal "Subur Kandungan", which consists of garlic (Allium sativum), temu mangga (Curcuma mangga), jeringau (Acorus calamus). The objective of this study was to determine the phytochemical content and antibacterial activity of A. sativum, C. mangga and A. calamus combination in water and ethanol solvent against Streptococcus aureus and Escherichia coli. There were three kinds of combinations with different composition ie first combination/C1 (36:36:28); second combination/C2 (40:30:30); third combination/C3 (35:40:45). Clindamycin was as a positive control. The phytochemical screening detected triterpenoids in both extracts, while alkaloids, flavonoids only in ethanol extracts. The highest inhibitory zone of both extracts to S. aureus and E. coli were discovered in C3 as strong and moderate inhibition. The most effective MIC value of water extract against S. aureus was found on C3 (9.76 x1010) while ethanol extract was obtained by C1 (5.9x108) at concentrations of 0.39% and MBC at 0.78%. The best MIC value of water extract against E. coli was found on C1 (1.08x1010) at a concentration of 25% and MBC at 50%, whereas ethanol extract was got on C3 (9.7x109) at 0.39% & MBC at 0.78%. It could be concluded that “Subur kandungan” herb recipes could be used as antibacterial drugs, which third combination/C3 in the ethanol solvent is the best treatment compare with others.
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